Cell Culture Scientist

KEY DUTIES AND RESPONSIBILITIES

Your duties as the Cell Culture Scientist will be varied however the key duties and responsibilities are as follows:

• As the Cell Culture Scientist, you will Establish and develop a comprehensive toolbox of host cell lines for manufacturing, ensuring suitability for diverse molecule types and GMP compliance.
• As the Cell Culture Scientist, you will generate, optimize, and validate stable mammalian cell lines to support therapeutic antibody production.
• As the Cell Culture Scientist, you will apply multiple host-engineering techniques, including genome-engineering technologies such as CRISPR, collaborating closely with engineering and molecular biology teams.
• As the Cell Culture Scientist, you will document processes rigorously, ensuring all activities are fully compliant with GMP regulations and supported by detailed records.
• As the Cell Line Sciences Scientist, you will communicate technical progress and challenges effectively across multidisciplinary teams, proposing practical solutions to achieve project milestones.

To be successful in your application to this exciting role as the Cell Culture Scientist we are looking to identify the following on your profile and past history:

• A Degree or higher level in a relevant scientific field (e.g., Biotechnology, Molecular Biology, Cell Biology).
• Proven experience generating stable cell lines for antibody and antibody-format expression in an industry setting.
• Solid understanding of genome-engineering techniques, such as CRISPR, and associated analytical methods.
• Hands-on experience with molecular biology techniques including ddPCR, cloning, and plasmid construction.
• Working knowledge of flow cytometry/FACS, single-cell cloning, and electronic lab systems.

Key Words: cell line development / stable cell line generation / mammalian expression systems / CHO cells / HEK
293 / recombinant protein expression / antibody expression / monoclonal antibodies / biologics development / upstream process development / cell engineering / genome editing / CRISPR-Cas9 / CRISPR engineering / host cell engineering / transfection / stable transfection / transient expression / gene integration / vector construction / plasmid design / plasmid engineering / gene cloning / molecular cloning / ddPCR / qPCR / PCR / DNA analysis / RNA analysis / protein expression analysis / western blot / ELISA / SDS-PAGE / protein purification / flow cytometry / FACS sorting / single-cell cloning / clone selection / clone screening / high-producing clone selection / cell banking / cell expansion / cell culture optimisation / mammalian cell culture / aseptic cell culture / suspension cell culture / serum-free media / bioreactor / shake flask culture / scale-up / upstream bioprocessing / process optimisation / cell line stability / cell viability analysis / productivity analysis / gene expression analysis / analytical assays / biologics manufacturing / GMP / GLP / regulated laboratory / documentation / batch records / electronic lab notebook / ELN / Snap Gene / Geneious / laboratory data management / scientific reporting / antibody therapeutics / therapeutic proteins / biopharmaceutical development / biotechnology / pharmaceutical R&D / life sciences.

"Hyper Recruitment Solutions Ltd (HRS) is an Equal Opportunities employer. We welcome applications from anyone who meets the role requirements. HRS exclusively supports the STEM sectors, combining recruitment expertise with scientific knowledge to help you advance your career".