Scientist Cryo - Electron Microscopy Center

Job Description

We are seeking an early career Scientist to work in our Cryo-EM Center e Children's Research Hospital.

The World's Brightest Minds Always Innovate

e Children's Research Hospital, we know what can be achieved when the brightest scientific minds face the fewest barriers. That's why we provide world-class facilities. State-of-the-art technologies. Extraordinary support. And a collaborative, bench-to-bedside environment where you can see, firsthand, how your science translates into survival for the children we serve. Quite simply, e, we encourage you to dream big and stop at nothing when it comes to finding cures and saving children.

The Cryo-EM Center e Children's Research Hospital is at the forefront of scientific discovery, supporting its scientists and investigators in their research pursuits. The Center provides its researchers with an entire Single Particle Analysis (SPA) solution and has rapidly progressed into the domain of Cryo-Electron Tomography. The Center has an exciting opportunity for a cryo-EM scientist who can contribute to world-class science relevant to basic research and impact cancer treatment.

Recently, the Center acquired cutting-edge instrumentation, including a Titan Krios G4 electron microscope (TEM) featuring a 'cold-FEG' electron source, a Selectris

X energy filter, and a Falcon 4i camera. The Center also houses a Titan Krios G3i and an Arctica microscope, both equipped with X-FEG, a Bio Quantum energy filter, and K3 camera. Furthermore, a 120kV Talos TEM with CETA 16M detector is routinely used for sample screening using negative staining technique. Delving into cryo-electron tomography, the Center has recently installed a FIB-Milling machine, Cross Beam 550.

For correlative light and electron microscopy under cryogenic conditions, an Airyscan LSM
900 with a Linkham stage is available.

The Center is well-equipped with a range of ancillary instruments for sample preparation, such as Thermo Fisher Vitrobot Mark IV, Leica EM GP2 Automatic Plunge Freezer, Leica ACE
600 Carbon Coater, Gatan Solarus II plasma cleaner, and PRIMO for micropatterning. The Center has several high-performance GPU workstations and generous storage for image processing. Moreover, the center is supported by St. Jude's high-performance computing facility with dedicated GPU and CPU nodes and a larger shared file system. The cryo-EM center team comprises four senior scientists with extensive experience in single particle analysis-cryo-EM.

• At least two years of experience in the vitrification of biological samples using semi-automated devices such as Vitrobot III or IV, Leica GP2 plunger.
• At least two years of experience independently operating an Arctica, a Glacios, or a Titan Krios electron microscope.
• Proficiency in SPA or tilt series data collection using programs such as EPU, TOMO5, and Serial
  
  EM.
• Hands-on experience with image processing and using one or two programs, such as RELION, cryo
  
  SPARC, EMAN2, SCIPION, and model analysis software, such as COOT and Chimera.
• Prior experience in a cryo
  
  EM facility setup is highly desirable.

• Support users with day-to-day operations of instruments such as Arctica and Krios microscopes.
• Prepare grids for samples provided by researchers across the St. Jude campus.
• Coordinate with the vendors for the repair and maintenance of the microscopes and ancillary equipment.
• Implement workflows that will help the researchers with their cryo-EM studies.
• Perform additional duties as assigned, such as performing FIB-milling. Training can be provided.
• Provide training to the users on instrument operation and sample preparation techniques.
• Stay well-aware about the progress in the field and should present new findings in journal clubs and department seminars.

• Bachelor's degree in relevant scientific area is required.

• Seven (7) years of relevant post-degree work experience is required with a Bachelor's degree.
• Five (5) years of relevant post-degree work experience is required with a Master's degree.
• Three (3) years of relevant post-degree work experience is required with a PhD.

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Plus, Memphians spend five hours less per year in their daily commute compared to the national average. Learn how you can "Have More in Memphis."

In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation…