Research Technician II

Overview

Fred Hutchinson Cancer Center is an independent, nonprofit organization providing adult cancer treatment and groundbreaking research focused on cancer and infectious diseases. Based in Seattle, Fred Hutch is the only National Cancer Institute-designated cancer center in Washington.

With a track record of global leadership in bone marrow transplantation, HIV/AIDS prevention, immunotherapy and COVID-19 vaccines, Fred Hutch has earned a reputation as one of the world's leading cancer, infectious disease and biomedical research centers. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services, and network affiliations with hospitals in five states.

Together, our fully integrated research and clinical care teams seek to discover new cures to the world's deadliest diseases and make life beyond cancer a reality.

At Fred Hutch we value collaboration, compassion, determination, excellence, innovation, integrity and respect. Our mission is directly tied to the humanity, dignity and inherent value of each employee, patient, community member and supporter. Our commitment to learning across our differences and similarities make us stronger. We seek employees who bring different and innovative ways of seeing the world and solving problems.

This research opportunity is with the McElrath Lab within the Vaccine & Infectious Disease Division. The technician will work under the direction of a Staff Scientist managing the molecular profiling team in support of the antigen‑specific B cell platform to help with the characterization of antigen‑specific B cell receptor repertoires in the context of HIV vaccine trials. In particular, they will assist in developing and implementing high‑throughput B cell receptor sequencing assays, including BCR PCR, Illumina NGS library preparation, CITE‑seq, micro neutralization screening, PCR cloning and transient transfection for monoclonal antibody production and functional screening of these monoclonals.

They will also be responsible for performing a variety of molecular and cellular biology techniques including multiplex PCR, single‑cell RT‑PCR, digital droplet PCR, maintenance of feeder cells under aseptic conditions, and RNA/DNA isolation. They will be involved in data management, interpretation and reporting on experimental results and assist with data presentation for lab meetings, conferences and manuscripts.

This person should be capable of maintaining detailed laboratory records, analyzing data, and reporting experimental results. They will interact closely with other laboratory staff to coordinate both up and downstream processing of samples and they need to be a good team player. They will assist in the development and maintenance of laboratory quality control (QC) methods and records, which may require adherence to GCLP (Good Clinical Lab Practice) standards.

• Perform molecular biology techniques: isolation of RNA/DNA from human lymphocytes for downstream analysis, gene expression by high density multiplex PCR, RT‑qPCR, RNA‑Seq, RT‑PCR amplification for targeted amplicon sequencing of B cell receptor genes (paired and unpaired), including indexing and library preparation for NGS Illumina platform, antibody cloning and purification, monoclonal antibody production by transient transfection.
• Perform tissue culture techniques: maintenance of cell lines including frozen inventories, transduction of feeder cells with retroviral and lentiviral vectors, virus neutralizations, cytotoxicity assays, in‑vitro cell manipulations, including peptide stimulations and co‑cultures.
• RNA and DNA isolation from whole blood, PBMC and tissue, Library preparation and indexing for Illumina sequencing platform.
• Operate and carry out basic maintenance and QC of laboratory equipment.
• Perform and/or optimize new assays and specimen processing methods as needed.
• May prepare stocks of HIV pseudo viruses for downstream neutralization.
• Prepare and maintain reagents, stocks, and cultures necessary to perform the work.
• Independently collect and analyze data.
• Drafting, editing and revising SOPs (standard operating…