Scientist II

Overview

We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Technologies team. This individual will be responsible for working in a dynamic team environment and will contribute to multiple work streams including but not limited to antibody screening using flow cytometry, hybridoma tissue culture and development, and recombinant protein expression.

The position requires extensive hands-on experience with flow cytometry, expertise in tissue culture, and an understanding of biochemistry and molecular biology.

• Responsible for the design and execution of experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FCS Express and Flow Jo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
• Responsible for transfection and expression of antibodies in cell lines, hybridoma tissue culture, and culture of various cell lines for testing. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques.
• Responsible for executing experiments using automated liquid handling and other automation equipment.

• Hands on experience designing, performing, and analyzing flow cytometry experiments, preferably on BD flow cytometers (LSRII, LSR Fortessa, Canto, Aria).
• Proficient in tissue culture techniques; including transfection of cells for protein expression.
• Software: BD FACS Diva, Flow Jo, FCS Express, and Microsoft Office Suite.
• Familiarity with assay automation.
• Familiarity with molecular biology (DNA preparation).
• Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.

A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 2-4 years of laboratory experience required. Flow cytometry and tissue culture experience required.