Senior Scientist; Genome Engineering - Genetics

Position Summary

The Senior Scientist in the Cremins Lab will be responsible for the oversight and implementation of specific research projects as well as participating in collaborative projects with other lab members and developing experimental protocols and experimental cell and molecular biology standards for the laboratory. The scientist will work on projects broadly encompassing genome engineering in the form of degrons, dTAG systems, Cas9, Cas
13, dCas, CRISPRoff, CRISPRon, and other new molecular technologies. The scientist will also lead experiments differentiating human iPSCs to neurons or organoids.

The ideal candidate will be versatile with both genome engineering and cell biology skills for new tech dev projects, standardizing genome engineering pipelines, providing feedback to trainees on construct building, and developing publication quality workflows for the in situ sequencing data analysis. The candidate will work as a subTeam leader over the progression of genome engineering projects and be responsible for maintaining and organizing lab records, training junior scientists on technical assays, working with the PI to prepare and review manuscripts, and helping with writing content for grant applications.

Will collaborate with the team to design, optimize, and standardize experimental and analysis pipelines to accelerate high-quality publications. Will review trainee data, provide feedback, and help ensure experiments are conducted in a single variable manner with positive and negative controls.

40 hours per week.

• Collaborates on designing, conducting and reporting of research projects.
• Responsible for deliverables of the Cremins lab genome engineering subTeam – including, but not limited to, conceptual selection of experimental strategies, protocol development, experimental design, validation, and training of other lab members in key bleeding edge experimental cell and molecular biology assays, including degrons/dTAG systems engineered in humans iPSCs, Cas
  13 RNA editing, dCas9 based CRISPRi, and CRISPRa, and other new cutting edge broadly relevant genome engineering technologies.
• Builds strong expertise in training in human iPSC differentiation to organoids and monolayer neurons as well as pilots experiments to facilitate neural aging in the lab.
• Leads multiple papers and major research papers at the center of the laboratories larger goals. Designs, conducts, assists and oversees experiments and original research in the area of neuronal genome regulation with a focus on elucidating structure-function relationships across scales of nucleus, synapses, circuits.
• Assists PI with NIH required data portal approvals, Grant and University-related paperwork, yearly RRPR reports for every one of the lab’s grants - including coordinating with the subTeam members to get the content assembled.
• Edits and gives feedback on postdoc and student proposal, grant submission, and manuscript drafts.
• Collaborates fluidly with computational experts to conduct efficient analyses leading to discovery in mammalian neuron genome structure-function data. In parallel, trains to achieve mastery and excellence in running code written by other members of the lab to analyze genomic and imaging data, for example calling DMLs in methylation data, calling compartments, TADs, subTADs, loops in Hi-C/Micro-C data, or peak calling of Cut&Run and Cut&Tag data.
• Mentors Junior trainees in scientific endeavors under the purview of the SRI’s expertise and trains new lab members on genome engineering and cellular differentiation.
• Oversees and writes established SOPs for new and existing molecular sequencing technologies – genome engineering, new and existing computational algorithms – iPSC differentiation to neurons, organoids, and assembloids.
• Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions (maintaining lab freezer maps and plasmid and antibody maps and reagent storage with excellence to keep a 25 person lab organized with high quality infrastructure).
• Performs other duties as assigned.

• Works in a…