Research Tech II--Xia Lab

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Job Location:

601 Elmwood Ave, Rochester, New York, 14642, United States of America.

Opening:
Worker Subtype:
Regular.

Time Type:
Full time. Scheduled Weekly

Hours:

40. Department: 400090 Pharmacology & Physiology Staff.

Work Shift:

UR - Day (United States of America). Range: UR URG 105 H. Compensation Range: $19.96 - $27.94 per hour.

Responsible for the maintenance, breeding, genotyping and organization of multiple transgenic mouse colonies. Additional responsibilities include completing established laboratory functions including cell culture and molecular and cell biological experiments. Will assist with overall operation of the laboratory including ordering supplies, making stock solutions, equipment maintenance, and training of lab personnel. Experience in cell culture and willingness to work with mice is required.

Ability to work in a lab team environment with a diverse group of personnel is essential.

• Mouse colonies management:
  Maintenance and breeding of multiple lines of transgenic mice. Cutting toes of P7 or younger mouse pups (or ear punches). Maintaining breeding records, setting up weekly breeding schedules, and detailed record-keeping of mouse genotypes in laboratory database.
• Molecular biology experiments and mice tail genotyping:
  Perform standard molecular biological experiments including cDNA purification, cloning, PCR amplification, restriction analysis, and PCR analysis of genomic DNA obtained from ear punches and tail snips.
• Cell biological experiments:
  Perform tissue sectioning, immunofluorescence, and other cell biological and biochemical experiments including preparing tissue or cultured cells for immunoprecipitation and/or western blotting experiments. Perfuse neonatal mouse pups, dissect different brain regions, and optimize assays as needed.
• General laboratory maintenance:
  Order supplies, maintain lab equipment, train lab workers, maintain lab notebooks, analyze data, design new protocols, guide undergraduate student workers, and provide technical assistance.
• Research Analysis, Presentation, Searches:
  Prepare literature searches, analyze and present findings to PI or in lab meetings.
• Biochemical Experiments:
  Perform biochemical immunoprecipitation studies and analyze data using SDS/PAGE and western blotting.
• Other projects and job duties as assigned.

• High school diploma or GED required.
• Associate's degree preferred.
• 1 year relevant experience in related field required; or equivalent combination of education and experience.

• Able to work with minimal supervision once trained, listen, convey, and exchange information with other lab personnel.
• Requires use of eyes, hands, fingers; ability to perform mathematical calculations using algebra and apply percentages, fractions, ratios.
• Ability to communicate effectively, follow instructions, record information accurately, excellent interpersonal skills.
• May entail prolonged periods of standing and walking and requires safe use of toxic chemicals.
• Daily interactions with Principal Investigator and other administrative and laboratory staff.

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