Principal Scientist, Molecular Biology

Overview

At Johnson & Johnson, we believe health is everything. Our strength in healthcare innovation empowers us to build a world where complex diseases are prevented, treated, and cured, where treatments are smarter and less invasive, and solutions are personal. Through our expertise in Innovative Medicine and Med Tech, we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow, and profoundly impact health for humanity.

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Job Function:
Discovery & Pre-Clinical/Clinical Development

Job Sub Function:
Biotherapeutics R&D

Job Category:
Scientific/Technology

All Job Posting Locations:
Spring House, Pennsylvania, United States of America

Job Description

About Innovative Medicine:
Our expertise in Innovative Medicine is informed and inspired by patients, whose insights fuel our science-based advancements. Visionaries like you work on teams that save lives by developing the medicines of tomorrow.

Join us in developing treatments, finding cures, and pioneering the path from lab to life while championing patients every step of the way. Learn more at

We are searching for the best talent for a Principal Scientist, Molecular Biology to be onsite in Spring House, PA.

Purpose:

The in Vivo Antibody Discovery group within Biologics Discovery is responsible for lead antibody generation from immunized animals with state-of-the-art technologies including single B cell sorting, hybridoma, display and microfluidics. In this key role, the Principal Scientist will lead a team responsible for
recovery of antibody sequences from selected hybridoma cells or single B cells
. The candidate is expected to work with automation experts to automate NGS workflow for efficient sequence recovery. In-depth knowledge and extensive experience in molecular cloning of BCR using various approaches including RT-PCR, Topo cloning, in-fusion cloning, multiplex NGS, 10X genomics are required. A strong understanding of B-cell recovery methods such as Hybridoma technology and primary B-cell cloning will also be important.

This is a critical function in a multifaceted Biologics Discovery organization committed to bringing the next generation of biotherapeutic molecules to patients. We are looking for a motivated candidate with scientific and technical expertise who can collaborate across discovery areas to bring value to our portfolio of innovative drug concepts. Proficiency to work within a highly coordinated, fast-paced environment will be a significant attribute.

If this sounds like you, come join our team!

You Will Be Responsible For

• Provide day-to-day in-person guidance and mentoring within the V-Gene Recovery (VGR) team
• Lead antibody sequence recovery from selected hits of diverse sources, including hybridoma, single B cells, immune repertoires, and in vitro display platforms.
• Execute and optimize molecular biology workflows, including Next-Generation Sequencing (NGS) and conventional Sanger sequencing, to enable efficient and accurate antibody discovery.
• Work with automation experts to automate and streamline NGS workflows, identifying opportunities for process improvements to enhance sequencing accuracy and efficiency.
• Manage laboratory operations by maintaining equipment, ensuring sufficient inventory of supplies, and overseeing the routine maintenance of NGS instruments and related laboratory equipment.
• Conduct comprehensive data analysis, interpret sequencing results, and generate detailed reports. Bioinformatics experience is a plus.
• Present research findings in internal meetings, clearly communicating scientific results and providing insights into data interpretation.
• Collaborate with multidisciplinary teams across functional areas to advance therapeutic programs, contributing scientific expertise to multiple projects in a dynamic and fast-paced environment.
• Document work accurately and promptly using Electronic Lab Notebook systems (e.g., Signal) and generate protocols and SOPs.
• Work closely with stakeholders and project managers to address operational needs and ensure seamless workflow execution.
• Demonstrate scientific curiosity and problem-solving skills, contributing to innovation and continuous improvement in molecular biology and sequencing workflows.

Qualifications / Requirements

Education and Years of

Experience:

• Minimum of a Bachelor’s degree is required
• PhD in Molecular Biology, Biomedical Engineering, Biochemistry or a related field is highly preferred

Required

• Minimum 6 years of experience in the pharmaceutical industry and/or academic setting
• Team management experience
• Proficiency in molecular biology techniques, including PCR, molecular cloning, and plasmid design.
• Expertise with DNA sequencing (NGS, 10x Genomics, Sanger) and sequence data analysis related to antibody discovery.

Preferred

• Familiarity with techniques for antibody repertoire analysis and single cell analysis.
• Knowledge of data management and visualization tools for large datasets, and knowledge with…