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College Student - Neuro-Oncology - Research

Job in Houston, Harris County, Texas, 77246, USA
Listing for: University of Texas MD Anderson Cancer Center
Apprenticeship/Internship position
Listed on 2026-03-15
Job specializations:
  • Research/Development
    Research Scientist
Job Description & How to Apply Below

Overview

A college student position is available in the department of Neuor-Oncology under the mentorship of Dr. Vinay Puduvalli. The college student will focus on conducting experiments (such as cell culture, plasmid preparation, etc.) to help other lab members with different projects. All duties and responsibilities are carried out in compliance with institutional policies, ethical research standards, and applicable federal and state regulations.

Learning

objectives
  • To learn how to culture and maintain human glioblastoma stem-like cells (GSCs) by using DMEM/F-12 culture medium without FBS. Activities will include
    1) Thawing, passing, and freezing GSC cells;
    2) checking cell viability with trypan blue;
    3) diluting the cells to seed them for experiments at appropriate density.
  • To learn how to conduct cell proliferation assay with GSCs upon drug treatment. Activities will include
    1) seed appropriate cell number onto 96-well plate;
    2) prepare drugs at required concentration;
    3) treat cells with drugs and incubate for the indicated time period;
    4) lyse the cells with Titer-Glo to perform Titer Glo Assay;
    5) analyze the data and plot graph for data presentation.
  • To learn how to transduce GSCs with lentiviral particles and select positive cells. Activities will include
    1) culture HEK 293T cells;
    2) transfect HEK
    293T cells with plasmids (i.e., pCMV-VSVG, pCMV-dR8.2dvpr, and pLenti

    Crispr-V2mO) using jetPRIME® transfection reagent (Polyplus);
    3) collect supernatant and concentrate the lentivirus;
    4) transduce GSCs and select target cells with selection markers.
  • To learn how to conduct western blot with GSC lysate and how to use Li-COR Odyssey CLX and Image Studio software to capture and analyze the image data.
  • Eligibility requirements

    Undergraduate student

    Position information

    Offsite work arrangements are subject to approval and may be modified or revoked at any time based on business needs, performance considerations, or regulatory requirements.

    This position may be responsible for maintaining the security and integrity of critical infrastructure, as defined in Section 113.001(2) of the Texas Business and Commerce Code and therefore may require routine reviews and screening. The ability to satisfy and maintain all requirements necessary to ensure the continued security and integrity of such infrastructure is a condition of hire and continued employment.

    It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law.

    It is policy also to provide, where applicable, equal opportunity information for applicants. See: http://(Use the "Apply for this Job" box below)..html

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