Associate Scientist - Mass Spectrometry Proteomics

Job

Employee Type: exempt full-time

Division:
Cancer Research Technology Program

Facility:
Frederick: ATRF

Location:

8560 Progress Dr, Frederick, MD 21701 USA

The Frederick National Laboratory is operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.

Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.

The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community. A major focus of the CRTP is the NCI RAS Initiative with the goal to discover new therapeutic interventions against RAS-driven cancers. In addition, the CRTP hosts the Nanotechnology Characterization Laboratory (NCL) which performs and standardizes preclinical efficacy and toxicity testing of nanoparticles intended for cancer therapeutics and diagnostics, and the Antibody Characterization Lab (ACL) which characterizes monoclonal antibodies or other renewable affinity binding reagents for use in cancer related research.

CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.

• Work independently and collaboratively with scientists to perform liquid chromatography-mass spectrometry (LC-MS)-based proteomics experiments, including: protein extraction/digestion, solid-phase extraction (SPE), immunoaffinity enrichment, etc. following established SOPs
• Help operate and maintain state-of-the-art LC-MS instrumentation (SCIEX 7500, Thermo TSQ Altis, Thermo Orbitrap Eclipse) and ensure high quality data generation for targeted proteomics
• Perform comprehensive data analysis using a variety of methods and software, such as Skyline, Excel, R, Python, etc.
• Formally present data to team members and collaborators
• Establish (design, test, and write) new or modify/optimize existing technical SOPs
• Document experimental results in a reproducible manner
• Assist with everyday lab operation, including but not limited to: maintaining lab inventories, ordering supplies, receiving packages, maintaining lab waste, facilitating engineer visits, communicating lab activities to supervisor, etc.
• Comply with all safety procedures, company policies, and established ways of work in the group

• Possession of Bachelor’s degree from an accredited college/university according to the Council for Higher Education Accreditation (CHEA) or four (4) years relevant experience in lieu of degree. Foreign degrees must be evaluated for U.S. equivalency
• In addition to the education requirement, a minimum of eight (8) years of related experience is required
• Both theoretical and hands-on experience in MS-based targeted (MRM and/or PRM) proteomics, including but not limited to: separation chemistry, sample preparation, LC-MS operation and troubleshooting, and data analysis
• Experience with performing large scale studies that require precise preparation (i.e., for quantitative work)
• Detail-oriented, highly-collaborative, self-motivated, and team-oriented
• Excellent verbal and written communication skills
• Ability to work on-site full time (8 hours/day, 5 days/week)
• Ability to obtain and maintain a security clearance

• M.S. degree in biochemistry, analytical chemistry or other related discipline from an accredited college or university according to the Council for Higher Education Accreditation
• 6 years of related post graduate MS-based proteomic experience
• Experience operating a SCIEX 7500 and/or Thermo TSQ Altis
• Experience operating a Waters ACQUITY UPLC M-Class, and/or Thermo Ulti Mate nano
  
  LC (i.e., microflow or nanoflow LC, respectively)
• Experience with Skyline software
• Experience with Covaris ultrasonicators and/or liquid…