Postdoctoral Fellow, Mass Spectrometry

The Team

The Biohub is a group of nonprofit research institutes that bring together scientists, engineers, and physicians with the goal of pursuing grand scientific challenges on 10- to 15-year time horizons. The Biohub focuses on understanding underlying mechanisms of disease and developing new technologies that will lead to actionable diagnostics and effective therapies.

Our Vision

• We pursue large scientific challenges that cannot be pursued in conventional environments
• We enable individual investigators to pursue their riskiest and most innovative ideas
• The technologies developed at the Biohub facilitate research by scientists and clinicians at our home institutions and beyond

Diversity of thought, ideas, and perspectives are at the heart of Biohub and enable disruptive innovation and scholarly excellence. We are committed to cultivating an organization where all colleagues feel inspired and know their work makes an important contribution.

Biohub Chicago seeks outstanding early-career scientists and engineers to join its Mass Spectrometry Omics platform as Postdoctoral Fellows in low-input and single-cell proteomics. This role offers a unique opportunity to work hands-on in a generously funded, highly collaborative environment at the intersection of technology development, biology, and disease-focused discovery.

Postdoctoral Fellows will be embedded in interdisciplinary teams developing and applying next-generation proteomics workflows for ultra-low-input and single-cell measurements. A central focus of this role is understanding how cell-to-cell proteome heterogeneity shapes the behavior of immune-competent organoid systems. The successful candidate will leverage advanced proteomics to interrogate cellular diversity, immune–tissue interactions, and emergent phenotypes that are inaccessible with bulk approaches.

This position provides strong mentorship alongside meaningful scientific independence within a team-science model that emphasizes collaboration across experimental, computational, and engineering domains. Projects align with the Biohub’s mission to uncover fundamental disease mechanisms and to develop transformative measurement technologies that accelerate biomedical research. Biohub Chicago is deeply committed to training scientists for impactful careers in academia, industry, and beyond.

• Design and execute low-input and single-cell proteomics experiments to investigate proteome heterogeneity in immune-competent organoid systems.
• Develop, optimize, and apply advanced mass spectrometry workflows for ultra-low-input samples, including method development, benchmarking, and quality control.
• Collaborate closely with interdisciplinary teams spanning tissue engineering, immunology, computation, and instrumentation to build and interrogate increasingly complex immune-organoid models.
• Analyze and interpret large-scale proteomics datasets using state-of-the-art computational pipelines, and communicate results clearly through figures, presentations, and internal discussions.
• Disseminate research outputs through high-impact publications, preprints, and open scientific resources, contributing to a culture of open and collaborative science.

• PhD in proteomics, analytical chemistry, biochemistry, immunology, or a related quantitative life-science field.
• 3 years of extensive hands-on experience with high-resolution mass spectrometry platforms (e.g., Bruker tims
  
  TOF, Thermo Orbitrap, or equivalent) and proteomics workflows.
• Demonstrated expertise in proteomics data analysis (e.g., DIA-NN, Frag Pipe, or related pipelines) and quantitative interpretation of complex datasets.
• Strong record of scientific productivity, creativity, and collaborative problem-solving in interdisciplinary research environments.
• Motivation to pursue innovative, independent research aligned with the Biohub’s mission and to thrive in a hands-on, team-science culture.

Nice to have

• Experience with low-input or single-cell sample preparation and isolation technologies (e.g., Cellen One, Tecan Uno).
• Background in immune cell biology, immune–tissue interactions, or organoid model systems.

The…