Senior Research Scientist; Protein Expression & Purification

Senior Research Scientist;
Protein Expression & Purification in Buffalo, NY

Build your future at Curia, where our work has the power to save lives.

The Senior Research Scientist, Protein Expression and Purification, is an integral part of the Curia team, contributing to our success by demonstrating expertise in both the theoretical and practical aspects of protein purification and characterization. Principal responsibilities are experimental design, conduct production & analysis experiments, maintain detailed records and generate standard operating procedures. Major components of the scientist's performance rating will be based on productivity, teamwork, analytical competence and experimental decision making.

Curia is a global contract development and manufacturing organization (CDMO) with over 30 years of experience partnering with pharmaceutical and biotech companies to provide life‑saving treatments to patients. At Curia, we are on a mission to advance our customers' therapies from curiosity to cure and ultimately to improve patients' lives.

• Generous benefit options (eligible first day of employment)
• Paid training, vacation and holidays (vacation accrual begins on first day of employment)
• Career advancement opportunities
• Education reimbursement
• 401K program with matching contributions
• Learning platform
• And more!
• Conduct protein purification with FPLC including affinity, ion exchange, HIC, etc.
• Conduct protein purification from
  
  E. coli, mammalian and/or insect cells.
• Run protein characterization including: HPLC, SDS-PAGE, native PAGE, western blot, DLS, capillary electrophoresis, and endotoxin assay.
• Be responsible for cell culture maintenance and transfections including: CHO, HEK 293, Sf9, and Sf21.
• Run molecular cloning including: PCR, DNA digestion, ligation, and mutagenesis.
• Present data (written and/or oral) to clients as necessary.
• Bachelor's degree in Biology or Biochemistry with a minimum of 5-8 years' related experience, or, a Master's degree in Biology or Biochemistry with a minimum of 3-6 years' related experience, or, a PhD with 0-3 years' related experience
• Extensive experience with cell culture and recombinant protein purification
• Prior experience with protein purification system such as AKTA, NGC, or similar is desired
• Skill of planning and organization of multiple projects simultaneously
• Ability of scientific data presentation and client communication
• Prior experience with molecular cloning and protein analysis is preferred
• Prior experience of purification of challenging proteins and troubleshooting skills are preferred
• Prior crystallization and cryo-EM experience is an asset

• Conduct protein purification with FPLC including affinity, ion exchange, HIC, etc.
• Conduct protein purification from
  
  E. coli, mammalian and/or insect cells.
• Run protein characterization including: HPLC, SDS-PAGE, native PAGE, western blot, DLS, capillary electrophoresis, and endotoxin assay.
• Be responsible for cell culture maintenance and transfections including: CHO, HEK 293, Sf9, and Sf21.
• Run molecular cloning including: PCR, DNA digestion, ligation, and mutagenesis.
• Present data (written and/or oral) to clients as necessary.

• Bachelor's degree in Biology or Biochemistry with a minimum of 5-8 years' related experience, or, a Master's degree in Biology or Biochemistry with a minimum of 3-6 years' related experience, or, a PhD with 0-3 years' related experience
• Extensive experience with cell culture and recombinant protein purification
  
  Prior experience with protein purification system such as AKTA, NGC, or similar is desired
• Skill of planning and organization of multiple projects simultaneously
• Ability of scientific data presentation and client communication

• Prior experience with molecular cloning and protein analysis is preferred
• Prior experience of purification of challenging proteins and troubleshooting skills are preferred
• Prior crystallization and cryo-EM experience is an asset

• Must pass a background check
• Must pass a drug screen
• May be required to pass Occupational Health Screening

Pay Range: $79,000/yr.

Education, experience, location and tenure may be…