Research Lab Tech , Genetics

Job Summary

Working independently, under general supervision, performs cutting‑edge research projects involving the use of CRISPR‑Cas9 high‑throughput screening to understand genetic disease and identify gene therapy strategies in the Sherwood lab ((Use the "Apply for this Job" box below).). This person will be in charge of all aspects of the projects for which they are responsible, which will include: stem cell culture; precision CRISPR‑Cas9 genome editing techniques; genomic analysis; molecular biology;

data analysis; and writing scientific papers to communicate salient findings. A strong academic background in genetics and CRISPR‑Cas9 is expected, and prior research experience at the undergraduate level or higher is required. Technicians are given autonomy to lead projects, and previously trained technicians have co‑authored journal articles and moved on to MD and PhD programs. While the primary responsibilities are wet‑lab experimental, the lab also has a strong computational component, so computational background is helpful but not essential.

Performs experimental laboratory work in the field of genomics, stem cell biology, and genome editing. Full project management requires integrated performance of all of these tasks on a daily basis along with organizational skills to balance project demands.

Work will include:

• Molecular biology including devising and carrying out plasmid cloning strategies.
• Cell culture including routine maintenance, genetic manipulation, and high‑throughput CRISPR‑Cas9 screening.
• Genome editing‑based screens including library production, testing, genomic library preparation and data analysis.
• Genomic procedures including ChIP‑seq, DNase‑seq, single cell RNA‑seq, PCR, and library preparation.
• Creative employment of novel laboratory techniques as the project demands.

• Maintain basic records of methodologies and test results in a log of work performed.
• Identify unusual findings and report such findings to supervisor.
• Maintain equipment and supplies in a neat and orderly manner.
• Maintain established department policies, procedures, objectives, quality assurance, safety, environmental and infection control.
• Maintain established hospital policies, procedures, and objectives for laboratory safety.

• B.S. in a biological/physical science required.
• Lab experience is required, experience with mammalian cell culture, molecular cloning, and genomic work is preferred.
• Must have a sound understanding of laboratory policies and procedures – usually acquired through one‑to‑three months on‑the‑job training.
• Must have good manual dexterity.
• Must demonstrate careful attention to detail.
• Must be able to maintain a neat and orderly working environment.

2200 The Brigham and Women's Hospital, Inc. is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.

To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran's Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at .