Research Assistant II-Endocrinology

Position Summary / Department Summary

We currently have one position open to study a wide range of research topics including cell‑based treatment of diabetes/obesity and intestinal stem cell differentiation. We are seeking highly motivated, curious, and enthusiastic individuals with excellent problem‑solving skills. Responsibilities include quantitative PCR, Western blot analysis, immunofluorescence staining, and flow cytometry as well as cell culture. Basic laboratory skills are required and experience in a previous lab is a plus.

Working occasional weekends may be warranted.

Intestinal stem cells, intestinal hormones, organoid culture

• Isolate intestinal crypts from patient biopsies when needed for new organoid lines
• Perform general cell culture work, including human intestinal organoids, mouse intestinal organoids, and human intestinal cell lines. Once sufficiently trained, will help with organoid differentiation experiments and infection of organoids with lentivirus for over‑expression and CRISPR knock out studies
• Perform general laboratory experiments (initially supervised): RNA isolation, PCR, quantitative PCR, western blots, immunofluorescence staining, and flow cytometry
• Use web‑based design tools to create novel CRISPR and over‑expression lentiviral plasmids
• Analyze and interpret experimental results to help determine the need for repeat studies
• Organize data into graphs for presentation which will be shown and discussed at weekly lab meetings
• Maintain an organized and thorough lab notebook that records protocols, results, and analysis
• Perform literature searches to help with the designing of new experiments (especially with using new cell culture materials, ie small molecules or viruses)
• Contribute to the writing and editing of new manuscripts
• Support general lab operations (ie, assist in purchasing supplies and maintaining stock solutions)

• A bachelor's degree in STEM is required, master's degree a plus but not required

• One year of work experience is required.
• Previous experience in a basic science laboratory.
• Have a background using basic mathematical and scientific principles to solve problems
• Have a background in using effective oral and written communication skills in a team setting

The posted pay range is Boston Children’s reasonable and good‑faith expectation for this pay at the time of posting.

Any base pay offer provided depends on skills, experience, education, certifications, and a variety of other job‑related factors. Base pay is one part of a comprehensive benefits package that includes flexible schedules, affordable health, vision and dental insurance, child care and student loan subsidies, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition and certain License and Certification Reimbursement, cell phone plan discounts and discounted rates on T‑passes.

Experience the benefits of passion and teamwork.