Postdoctoral Fellow

Overview

Cryo-EM Postdoc Positions in the Hinshaw Lab  are seeking highly motivated postdoctoral fellow candidates interested in structure-function studies of dynamin proteins. We are investigating the dynamic properties of dynamin proteins by cryo-EM, biochemical and fluorescent microscopy approaches. Dynamin family members are large GTPases involved in membrane fission and fusion events in the cell. Mutations in dynamin have been shown to cause peripheral neuropathies, Charcot–Marie–Tooth disease (CMT), centronuclear myopathy (CNM) and Hereditary Spastic Paraplegia (HSP).

Other dynamin family members are involved in processes including division and fusion of mitochondria (Drp1, Mfn, Opa1). Mutations in Opa1 (Optic Atrophy) are a leading cause of childhood blindness while mutations in Mfns (mitofusins) lead to CMT and hereditary motor and sensory neuropathy (HMSN). We are currently exploring the structure and function of dynamin, mitofusins, and Opa1, and relating specific mutations to a disease state.

The laboratory has excellent resources for cryo-electron microscopy, cryo-electron tomography, and fluorescent microscopy. Our group has access to two Titan Krios-GIF/K3, Glacios-K3, TF20-K2, and two T12 microscopes. For cryo-ET, we have access to an Aquilos cryo

FIB milling and Leica CLEM microscopes.

• Contribute to structure-function studies of dynamin family proteins using cryo-EM, biochemical methods, and fluorescence microscopy.
• Assist with data collection, processing, and interpretation of cryo-EM images and collaborate with team on project planning and results presentation.

• Expertise in protein biochemistry (protein expression and purification) and cryo-EM methods including image processing.

NIH postdoc Requirements are posted on the following website: https://(Use the "Apply for this Job" box below). Applicants should send a cover letter, briefly describing previous research experience and future goals, a CV, and contact information for three professional references to: jenny.hinshaw

Jenny Hinshaw, PhD
Director, Multi-Institute Cryo-EM Facility (MICEF)
Chief, Laboratory of Molecular Biology
-hinshaw